Data/hora: 02/06/2024 16:21:27

Provedor de dados: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia	
País: 	Brazil	
Título: 	ELISA indireto para detecção de IgG antivírus da doença de Newcastle em soro de codorna	
Autores: 	Oliveira,D.D.; Folgueras-Flatschart,A.V.; Flatschart,R.B.; Resende,J.S.; Abreu,J.T.; Martins,N.R.S.	
Data: 	2007-10-01	
Ano: 	2007	
Palavras-chave: 	Codorna japonesa; ELISA; IgG; Doença de Newcastle.	
Resumo: 	An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100%). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific IgG in japanese quail sera, with the advantage of high sensitivity and automation.	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Português	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500040	
Editor: 	Universidade Federal de Minas Gerais, Escola de Veterinária	
Relação: 	10.1590/S0102-09352007000500040	
Formato: 	text/html	
Fonte: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.59 n.5 2007	
Fonte: 	info:eu-repo/semantics/openAccess