Data/hora: 10/06/2024 06:58:32

Provedor de dados: | 45
País: | Brazil
Título: | Sphingosine 1-phosphate promotes activation of aprine preantral follicle in vitro
Autores: | Nóbrega Jr.,J.E.; Rossetto,R.; Matos,M.H.T.; Chaves,R.N.; Magalhães,D.M.; Lima-Verde,I.B.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R.; Gonçalves,P.B.D.; Oliveira,J.F.C.
Data: | 2014-08-01
Ano: | 2014
Palavras-chave: | Goat; Preservation; Preantral follicles; Sphingosine 1-phosphate; Follicular activation.
Resumo: | This study describes the effect of sphingosine 1-phosphate (S1P) for development of preantral follicle, therefore the activation and follicular viability of caprine follicles cultured in vitro. Ovarian fragments were cultured for 1 or 7 days in Minimum Essential Medium with different S1P concentrations (0, 1, 10, 50, 100 or 200ng/mL). All ovarian fragments were processed for histological analysis in optical microscopy, transmission electron microscopy and fluorescence analysis. The treatment using 1ng/mL of S1P was able to maintain the percentage of normal follicles with the progression of the culture from day 1 to 7. At end of the 7-day culture period there was a significant reduction (P&lt;0.05) in the percentage of primordial follicles in all groups treated with S1P, compared with fresh control (FC) and Control Culture (CC), which was followed by an increase of activated follicles (intermediary, primary and secondary). In addition, the culture for 7 days with media supplemented with S1P with 1ng/mL preserved the ultrastructure of organelles and kept the preantral follicular viability when evaluated by fluorescence microscopy. In conclusion, after 7 days of culture, the 1ng/mL of S1P activates the development of preantral caprine follicles, cultured in situ and maintains the oocitary and follicular viability.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000400977
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-6455
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.66 n.4 2014
Fonte: | info:eu-repo/semantics/openAccess