Data/hora: 01/06/2024 18:42:18

Provedor de dados: | 45
País: | Brazil
Título: | Ocorrência de apoptose em leucócitos no esfregaço de sangue periférico e em sincícios na infecção in vivo pelo vírus da cinomose canina
Autores: | Moro,L.; Alves,C.M.; Santos,F.G.A.; Nunes,J.E.S.; Carneiro,R.A.; Vasconcelos,A.C.
Data: | 2003-02-01
Ano: | 2003
Palavras-chave: | Cão; Apoptose; Cinomose; Vírus.
Resumo: | The aim of this paper is to report the occurrence of apoptosis in peripheral blood leukocytes and in syncytia induced by experimental infection of dogs with canine distemper virus. Blood was collected from nine puppies that were inoculated with the Snyder Hill strain of CDV, and stained with May Grunwaldt-Giemsa. Dogs were necropsied and retropharyngeal lymph nodes were collected and processed for paraffin embedding. Five micrometers thick sections were stained with hematoxylin-eosin and methyl green pyronin. Other sections were submitted to TUNEL reaction for in situ detection of apoptosis, and immunohistochemistry to detect CDV antigen. Immunohistochemistry confirmed the presence of viral antigen. Syncytia were present in all retropharyngeal lymph nodes. Several syncytia contained apoptotic nuclei as detected by hematoxylin-eosin and methyl green pyronin stains. Apoptotic nuclei were labeled inside syncytia by the TUNEL reaction. Blood smear analyses indicated higher proportion of apoptotic leukocytes in the peripheral blood of infected dogs.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Português
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000100018
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352003000100018
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.55 n.1 2003
Fonte: | info:eu-repo/semantics/openAccess