Data/hora: 12/06/2024 17:18:13

Provedor de dados: | 45
País: | Brazil
Título: | Profile and regulation of annexin II expression during early embryogenesis in cattle
Autores: | Costa,L.F.S.; Machado,M.S.N; Oliveira,J.F.C.; Silva,J.C.; Loguercio,R.S.; Gonçalves,P.B.D.
Data: | 2007-12-01
Ano: | 2007
Palavras-chave: | Cattle; Annexin II; Embryo development.
Resumo: | The presence of annexin II (Ann-II) during the initial stages of bovine embryo development and the regulation of Ann-II expression by retinol and insulin-like growth factor I (IGF-I) were studied. Bovine embryos at different stages of development were produced in vitro on Synthetic Oviductal Fluid (SOF) medium (control group), SOF supplemented with retinol (retinol group; 0.1ng/ml), or IGF-I (IGF-I group; 10ng/ml). The embryos were processed for mRNA extraction, cDNA production and polymerase chain reaction (PCR) using Ann-II-specific oligonucleotides. Ann-II was detected in all stages of early embryo development, except for the 16-cell stage. The blastocyst rates were significantly higher (P&lt;0.05) in the group supplemented with retinol (37.8%, 45/119) during in vitro embryo culture (IVC) than in those cultured in SOF (20.5%, 24/117) or SOF with IGF-I (25.8%, 24/93). Semiquantitative analysis of Ann-II expression in embryos produced in medium supplemented with IGF-I or retinol revealed a lower expression of this gene when compared with embryos cultured in SOF (P&lt;0.05). The Ann-II expression was not different in embryos cultured in the presence of retinol and IGF-I. The presence of retinol increased the production of embryos in vitro by decreasing the expression of Ann-II in early-stage of bovine embryo.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600023
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352007000600023
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.59 n.6 2007
Fonte: | info:eu-repo/semantics/openAccess