Data/hora: 11/06/2024 01:20:34

Provedor de dados: | 45
País: | Brazil
Título: | The use of mice as animal model for testing acute toxicity (LD-50) of toxic shock syndrome toxin
Autores: | Dias,R.S.; Carmo,L.S.; Heneine,L.G.D.; Rocha,P.H.; Barbosa,C.F.; Rodrigues,R.J.; Linardi,V.R.
Data: | 2009-02-01
Ano: | 2009
Palavras-chave: | Mice; TSST-1; Acute toxicity (LD-50); Staphylococcus.
Resumo: | Acute toxicity test (LD-50) using toxic shock syndrome toxin (TSST-1) was tested in BALB/c, C57BL/6 and Swiss mice. Animals (n = 10) were intraperitoneally injected with TSST-1 (0.01-10.0µg/mouse) followed 4h later by potentiating dose of lipopolysaccharide (75.0µg of LPS - E. coli O111:B4) and cumulative mortality was recorded over 72h. Control animals received either TSST-1 or LPS alone. The data were submitted to qui-Square test and acute toxicity test was calculated by probit analysis (confidence limits expressed as µg toxin/kg). BALB/c mice was the most sensitive (20.0µg/kg, 95% confidence limits: 9.0-92.0) followed by C57BL/6 (38.5µg/kg, 95% confidence limits: 9.11- 401.6). Data from Swiss mice was not conclusive, indicating only low sensitivity. Selection of the animal model and standardization of the experiment are fundamental for the development of serum neutralization tests used for final quality control of vaccine production.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100024
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352009000100024
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.61 n.1 2009
Fonte: | info:eu-repo/semantics/openAccess