Data/hora: 10/06/2024 18:02:07

Provedor de dados: | 45
País: | Brazil
Título: | Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate
Autores: | Fonseca,J.F.; Batista,R.I.T.P.; Souza-Fabjan,J.M.G.; Oliveira,M.E.F.; Brandão,F.Z.; Viana,J.H.M.
Data: | 2018-10-01
Ano: | 2018
Palavras-chave: | Embryo survival; Embryo transfer; Goat; Slow freezing.
Resumo: | ABSTRACT The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to -6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from -6 C to -32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were maintained in holding medium (37ºC). Fresh and frozen-thawed embryos were transferred at day 7 post-estrus to 30 recipients. Kidding and kid born rates were similar (P&gt; 0.05), respectively, for recipients receiving fresh (66.7% or 10/15; 55.2% or 16/29) or frozen-thawed (60% or 9/15; 51.7% or 15/29) embryos. The cryopreservation of goat embryos using slow-freezing protocol and 1.5MEG resulted in similar efficiency rates of fresh embryos.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501489
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-4162-10196
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.70 n.5 2018
Fonte: | info:eu-repo/semantics/openAccess