Data/hora: 16/06/2024 06:35:56

Provedor de dados: | 45
País: | Brazil
Título: | Histological evaluation and E-cadherin and β-catenin expression in kidney of dogs submitted to renal ischemia and reperfusion after chlorpromazine administration
Autores: | Menezes,L.B.; Fioravanti,M.C.S.; Oliveira,F.A.; Silva,M.S.B.; Franco,L.G.; Sales,T.P.; Andrascko,M.M.; Guimarães,L.L.B.; Miguel,M.P.; Araújo,E.G.
Data: | 2017-10-01
Ano: | 2017
Palavras-chave: | Acute renal failure; Adherens junctions; Cell adhesion; Kidney tubular necrosis.
Resumo: | ABSTRACT Renal ischemia can be associated with some urological procedures, such as renovascular surgery or kidney transplantation, that are often followed by acute renal failure. The aim of this study was to verify the E-cadherin and β-catenin localization in canine kidney in different times of renal ischemia and reperfusion after chlorpromazine application. Twelve dogs were randomly distributed equally into two groups. GroupA with ischemia and reperfusion without chlorpromazine and groupB with ischemia and reperfusion treated by chlorpromazine. GroupB received intravenous chlorpromazine, 15 min before the artery obstruction, which lasted 1 hour. After this period, the clamps in the renal arteries were released and the organ remained in reperfusion for 2 hours. In each group, anti-E-cadherin and anti-β-catenin antibodies were made in six tissue samples from renal parenchyma. E-cadherin and β-catenin are differentially expressed in segments from cortex and medulla in dog’s kidneys and the use of chlorpromazine did not alter the expression of both proteins. Occlusion of the left renal artery in dogs causes morphological alterations mainly in proximal convoluted tubules, beginning 30min after the start of ischemia and being aggravated after two hours of reperfusion. These results reveal that chlorpromazine did not change kidneys’ histological aspect nor E-cadherin and β-catenin expression.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501206
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-4162-9395
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.69 n.5 2017
Fonte: | info:eu-repo/semantics/openAccess