Data/hora: 11/06/2024 02:46:27

Provedor de dados: | 45
País: | Brazil
Título: | Identification of Staphylococcus spp. isolated during the ripening process of a traditional minas cheese
Autores: | Borelli,B.M.; Lacerda,I.C.A.; Brandão,L.R.; Vianna,C.R.; Ferreira,M.C.; Gomes,F.C.O.; Carmo,L.S.; Heneine,L.G.D.; Rosa,C.A.
Data: | 2011-04-01
Ano: | 2011
Palavras-chave: | Canastra Minas cheese; Staphylococcus; Enterotoxin.
Resumo: | The population dynamics of Staphylococcus spp. was studied during the ripening of Canastra Minas cheese at three farms located in the State of Minas Gerais, Brazil. The presence of coagulase (coa), thermonuclease (nuc), and enterotoxin (sea, seb, sec, and sed) genes was investigated in Staphylococcus strains isolated during the 60-day cheese-ripening period. The presence of the staphylococcal enterotoxins A, C, and D was also investigated in the cheese samples. Cheese samples that were matured for 0, 7, 15, 30, and 45 days presented staphylococci counts from 10³ to 10(8)cfu/g. All isolates considered coagulase-positive by physiological tests had the coa gene. However, no association was observed between the results obtained with biochemical tests and those obtained by PCR using gene-specific primers for coagulase-negative strains. Coagulase and thermonuclease genes occurred simultaneously in 41.3% of Staphylococcus spp. tested. None of the investigated Staphylococcus strains expressed enterotoxins SEA, SEB, SEC, and SED. Enterotoxins A, C, and D were not detected in any of the cheese samples.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000200028
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352011000200028
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.63 n.2 2011
Fonte: | info:eu-repo/semantics/openAccess