Data/hora: 11/06/2024 12:10:50

Provedor de dados: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia
País: | Brazil
Título: | Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams
Autores: | Costa,L.F.; Nozaki,C.N.; Lira,N.S.C.; Antunes,J.M.A.P.; Xavier,M.N.; Costa,E.A.; Paixão,T.A.; Santos,R.L.; Megid,J.
Data: | 2013-02-01
Ano: | 2013
Palavras-chave: | Brucella ovis; Species-specific; Nested PCR; Semen; Urine.
Resumo: | The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a genus-specific PCR. Fourteen rams were experimentally infected with the Brucella ovis REO 198 strain and samples of semen and urine were collected every week up to 180 days post infection. Out of 83 semen samples collected, 42 (50.6%) were positive for the species-specific nested PCR, and 23 (27.7%) were positive for the genus-specific PCR. Out of 75 urine samples, 49 (65.3%) were positive for the species-specific nested PCR, whereas 11 (14.6%) were genus-specific PCR positive. Species-specific nested PCR was significantly more sensitive (P&lt;0.001) than the genus-specific PCR in semen and urine from experimentally infected rams. In conclusion, the species-specific nested PCR developed in this study may be used as a diagnostic tool for the detection of B. ovis in semen and urine samples from suspected rams.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100009
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352013000100009
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.65 n.1 2013
Fonte: | info:eu-repo/semantics/openAccess