Data/hora: 08/06/2024 16:10:46

Provedor de dados: 	46	
País: 	Argentina	
Título: 	In vitro apprach to the study of chronic exposure to low does of X-rays	
Autores: 	Ponzinibbio,M.V; Peral-García,P; Seoane,A.	
Data: 	2011-12-01	
Ano: 	2011	
Palavras-chave: 	Apoptosis; Comet assay; In vitro; Ionizing radiation; Low dose.	
Resumo: 	The present research was undertaken in order to study the effect of repeated low doses of radiation on two different cell lines from the same species. An in vitro model test was developed to avoid the influence of the confounding factors affecting epidemiological studies and to simulate a chronic exposure (50 mSv of x-rays during ten consecutive days). Comet assay and early apoptosis were analyzed immediately after exposure and after chronic irradiation. Sequential irradiation induced an increase of cells showing DNA damage. Index Damage was higher than that of the controls in both cell lines immediately after exposure and after chronic irradiation. These differences between exposed and control cells were statistically significant only for the transformed cell line after chronic irradiation (p&lt;0.001). Significantly higher levels of apoptosis were scored after chronic exposure in both cells lines (p&lt;0.001). The induction of DNA damage and apoptosis in hamster cells by chronic exposure to low dose ionizing radiation was demonstrated. Cell types reacted differently to chronic exposure. Though further investigation is needed to understand the mechanisms of radiation effects on chronic low-dose-exposed cell populations, cellular type should be taken into account in the design of in vitro experiments to understand low-dose-irradiation effects.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000200004	
Editor: 	Sociedad Argentina de Genética	
Formato: 	text/html	
Fonte: 	BAG. Journal of basic and applied genetics  v.22 n.2 2011	
Fonte: 	info:eu-repo/semantics/openAccess