Data/hora: 08/06/2024 10:29:45

Provedor de dados: 	47	
País: 	Argentina	
Título: 	Seasonal variations in the heterologous binding of viscacha spermatozoa: A scanning electron microscope study	
Autores: 	Aguilera Merlo,Claudia; Muñoz,Estela; Dominguez,Susana; Fóscolo,Mabel; Scardapane,Luis; de Rosas,Juan Carlos.	
Data: 	2005-12-01	
Ano: 	2005	
Palavras-chave: 	Viscacha (Lagostomus maximus maximus); Spermatozoa; Heterologous binding; Seasonal variations.	
Resumo: 	Seasonal changes in the reproductive activity of the adult male viscacha (Lagostomus maximus maximus) were investigated during the annual reproductive cycle. Assays of heterologous in vitro binding between compatible gametes were used to evaluate the ability of viscacha spermatozoa to achieve primary binding during its annual reproductive cycle. Sperm were collected by mincing cauda epididymis in HECM-3 medium and the sperm concentration and motility were evaluated. Cumulus-free and zona-free oocytes obtained from superovulated hamsters were inseminated in vitro with capacitated sperm suspensions, incubated at 37ºC, 5% CO2 for 3 h, and then processed for studies by scanning electronic microscopy. Statistical analysis was used to compare the quantitative differences. The number of spermatozoa significantly decreases during the regression period, while sperm motility was progressive speed in both periods. During the active period elevated sperm binding to cumulus-free and zona-free oocytes was observed, while the binding during the regression period decreased drastically. In both periods, oocyte microvilli covered sperm heads and tails. These results suggest that the ability of viscacha spermatozoa to participate in gamete recognition is profoundly affected. This would likely be related to different functional stages of the spermatozoa and their epididymal microenvironment during the annual reproductive cycle of viscacha.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300001	
Editor: 	Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)	
Formato: 	text/html	
Fonte: 	Biocell  v.29 n.3 2005	
Fonte: 	info:eu-repo/semantics/openAccess