Data/hora: 02/06/2024 11:52:03

Provedor de dados: 	Biocell	
País: 	Argentina	
Título: 	Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells	
Autores: 	R odriguez,Andrea P.; Tsujigiwa,Hidetsugu; Gunduz,Mehmet; Cengiz,Beyhan; Nagai,Noriyuki; Tamamura,Ryo; Borkosky,Silvia S.; Takagi,Tohru; Inoue,Miho; Nagatsuka,Hitoshi.	
Data: 	2009-04-01	
Ano: 	2009	
Palavras-chave: 	MDPC-23 cells; KUSA/A1 cells; Diffusion chamber; RT-PCR analysis; Immunohistochemical analysis.	
Resumo: 	Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100005	
Editor: 	Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)	
Formato: 	text/html	
Fonte: 	Biocell  v.33 n.1 2009	
Fonte: 	info:eu-repo/semantics/openAccess