Data/hora: 04/06/2024 16:55:09

Provedor de dados: | 47
País: | Argentina
Título: | Influence of 2-methoxyestradiol on MCF-7 cells: An improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels
Autores: | Joubert,Annie; Marais,Sumari; Maritz,Christine.
Data: | 2009-04-01
Ano: | 2009
Palavras-chave: | Breast carcinoma; Mitosis; Apoptosis.
Resumo: | Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 &amp;#956;M 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. The study proposes that the influence of 2-methoxyestradiol on the expression of these proteins may be time- and cell type dependent and thus not evident during the mitotic block observed. Investigation of the regulation of the B-cell lymphoma 2 family will allow researchers to consider signaling pathways for diseases where apoptosis can potentially be controlled.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100007
Editor: | Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
Formato: | text/html
Fonte: | Biocell  v.33 n.1 2009
Fonte: | info:eu-repo/semantics/openAccess