Data/hora: 04/06/2024 01:15:10

Provedor de dados: | 48
País: | Chile
Título: | Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
Autores: | Bae,Seunghee; Jung,Younjung; Choi,Yeong Min; Li,Shunhua.
Data: | 2015-01-01
Ano: | 2015
Palavras-chave: | Er-Miao-San MMP1 Human dermal fibroblasts TNF-&amp;#945; NF-kB.
Resumo: | BACKGROUND: Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown. RESULTS: This study investigated the effects of EMS on TNF-&amp;#945;-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-&amp;#945;-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IkB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-kB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-kBbystabilizing IkB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-kB-regulated genes such as &amp;#921;L-1&amp;#946; and IL-8. Moreover, EMS effectively inhibited TNF-&amp;#945;-induced expression of MMP-1 via the NF-kBpathway. CONCLUSIONS: Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100009
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.48 2015