Data/hora: 08/06/2024 16:56:03

Provedor de dados: | 48
País: | Chile
Título: | Ventricular Myosin Light Chain-2 Gene Expression in Developing Heart of Chicken Embryos
Autores: | GHATPANDE,SATISH; SHAFIQ,SAIYID; SIDDIQUI,M.A.Q.
Data: | 2001-01-01
Ano: | 2001
Palavras-chave: | Cardiogenesis; Cardiomyopathy; Myosin light chains; Tissue culture.
Resumo: | Recent gene knock-out studies in mice have suggested that ventricular myosin light chain-2 (vMLC2) has a role in the regulation of cardiogenic development and that perturbation in expression of vMLC2 is linked to the onset of dilated cardiomyopathy. In an attempt to develop an avian model for such studies, we examined the expression pattern of vMLC2 in chicken embryos at various stages and analyzed the effect of antisense oligonucleotide-mediated interference of vMLC2 function in cultures of whole embryos. Our results showed vMLC2 to be a specific marker for ventricular chamber throughout chicken embryonic development and antisense vMLC2 treatment of primitive streak stage (stage 4) embryos to produce pronounced dilation of heart tube with severe deficiency in formation of striated myofibrils. Further studies with antisense mRNA techniques of whole embryo cultures should, therefore, be useful to evaluate the role of vMLC2 and other putative regulatory factors in cardiac myofibrillogenesis
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100009
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.34 n.1 2001