Data/hora: 11/06/2024 03:33:24

Provedor de dados: | Biological Research
País: | Chile
Título: | Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae
Autores: | GÖTTE,MARTIN; STADTBÄUMER,ANDREA.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | Vesicular transport; SNARE; Syntaxin; Endosomal trafficking; Vacuole protein sorting; PEP12.
Resumo: | The molecular mechanisms of vesicular protein transport in eukaryotic cells are highly conserved. Members of the syntaxin family play a pivotal role in the membrane fusion process. We have expressed rat syntaxin 6 and its cytoplasmic domain in wild-type and pep12 mutant strains of Saccharomyces cerevisiae to elucidate the role of the syntaxin 6-dependent vesicular trafficking step in yeast. Immunofluorescence microscopy revealed a punctate, Golgi-like staining pattern for syntaxin 6, which only partially overlapped with Pep12p in wild-type yeast cells. In contrast to Pep12p, syntaxin 6 was not mislocalized to the vacuole upon expression from 2 micron vectors, which might be attributed to conserved sorting and retention signals. Syntaxin 6 was not capable of complementing the sorting and maturation defects of the vacuolar hydrolase CPY in pep12 null mutants. No dominant negative effects of either syntaxin 6 or syntaxin 6deltaC overexpression on CPY sorting and maturation were observed in wild-type yeast cells. We conclude that syntaxin 6 and Pep12p do not act at the same vesicular trafficking step(s) in yeast and higher eukaryotes
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300009
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.35 n.3-4 2002