Data/hora: 11/06/2024 12:19:31

Provedor de dados: | Biological Research
País: | Chile
Título: | Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
Autores: | WILHELM,VIVIAN; SOZA,CRISTIÁN; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D.T.
Data: | 2005-01-01
Ano: | 2005
Palavras-chave: | Piscirickettsia salmonis; Hsp60; Hsp70; GroEL; DnaK; Immune response.
Resumo: | We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.38 n.1 2005