Data/hora: 01/06/2024 18:06:24

Provedor de dados: | Biological Research
País: | Chile
Título: | IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
Autores: | JAIMOVICH,ENRIQUE; CARRASCO,MARIA ANGELICA.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | Excitation-transcription; Inositol trisphosphate; Mitogen-activated kinases; CREB; Early genes; Dihydropyridine receptors.
Resumo: | Potassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave along the myotube showing a distinct calcium transient at the level of cell nuclei. Due to the presence of IP3 receptors both in the SR (A-band region) and in the nuclear envelope, these two events appear to be related; iii) Phosphorylation of mitogen activated kinases (ERK 1/2) and of the transcription factor CREB (30 s-10 min), as well as expression of the early genes c-fos, c-jun and egr-1 mRNA (5-15 min). Several independent pieces of evidence, including results obtained using inhibitors specific for individual steps, allowed us to connect these in a sequential manner. As the same type of signaling cascade can be triggered by oxidants, neurotransmitters and hormones, the ensemble of results allows us to propose a general model to describe signaling events that link membrane stimulation to regulation of gene transcription in skeletal muscle cells
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200010
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.35 n.2 2002