Data/hora: 10/06/2024 16:15:12

Provedor de dados: | 48
País: | Chile
Título: | Integrin-&amp;#946;1, not integrin-&amp;#946;5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
Autores: | Zeng,Qiangcheng; Guo,Yong; Liu,Yongming; Li,Ruixin; Zhang,Xinchang; Liu,Lu; Wang,Yang; Zhang,Xizheng; Zou,Xianqiong.
Data: | 2015-01-01
Ano: | 2015
Palavras-chave: | Tensile strain; Osteoblast; Extracellular matrix; Integrin; SiRNA.
Resumo: | BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-&amp;#946; (&amp;#946;1, &amp;#946;5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-&amp;#946; in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-&amp;#946;1 siRNA or integrin-&amp;#946;5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µ&amp;#949;) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-&amp;#946;1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-&amp;#946;5 siRNA had little effect on the osteoblastic differentiation induced by thestrain. At thesametime, theresultofECM formation promoted by the strain, was similar to the osteoblastic differentiation. CONCLUSION: Integrin-&amp;#946;1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-&amp;#946;5 is not involved in the osteoblasts response to the tensile strain.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.48 2015