Data/hora: 10/06/2024 18:22:13

Provedor de dados: | 48
País: | Chile
Título: | Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
Autores: | LANZA,HENRIQUE; AFONSO-CARDOSO,SANDRA R; SILVA,ADRIANO G; NAPOLITANO,DANIELLE R; ESPÍNDOLA,FOUED S; PENA,JANETHE D.O; SOUZA,MARIA A.
Data: | 2004-01-01
Ano: | 2004
Palavras-chave: | Calcium; Leishmania major; Macrophage; Magnesium; Nitric oxide.
Resumo: | Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine macrophages while Mg2+ seemed to increase parasite proliferation. These finding may help to understand the events involved in host cells' clearance of this pathogen
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300004
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.37 n.3 2004