Data/hora: 08/06/2024 02:41:08

Provedor de dados: | Biological Research
País: | Chile
Título: | Characterization of mitochondrial iron uptake in HepG2 cells
Autores: | MAZARIEGOS,DORA INÉS; CABANTCHIK,Z. IOAV; CASSELS,BRUCE K; NÚÑEZ,MARCO TULIO.
Data: | 2006-01-01
Ano: | 2006
Palavras-chave: | Mitochondria; Iron homeostasis; RPA; HepG2 cells.
Resumo: | There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.39 n.1 2006