Data/hora: 04/06/2024 01:35:20

Provedor de dados: 	48	
País: 	Chile	
Título: 	Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus	
Autores: 	Thao,Nguyen Phuong; Luyen,Bui Thi Thuy; Koo,Jung Eun; Kim,Sohyun; Koh,Young Sang; Cuong,Nguyen Xuan; Nam,Nguyen Hoai; Van Kiem,Phan; Kim,Young Ho; Van Minh,Chau.	
Data: 	2015-01-01	
Ano: 	2015	
Palavras-chave: 	Protoreaster nodosus Starfish IL-12 p40 IL-6 TNF-&amp;#945; LPS-stimulated BMDCs Anti-inflammatory.	
Resumo: 	BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-&amp;#945;) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). RESULTS: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ±0.01 to 26.19 ±0.64 &amp;#956;g/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S)5&amp;#945;-cholestane-3&amp;#946;,6&amp;#945;,7&amp;#945;,8&amp;#946;,15&amp;#945;,16&amp;#946;,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 &amp;#956;M), and for (25S)5&amp;#945;-cholestane-3&amp;#946;, 6&amp;#945;,8&amp;#946;, 15&amp;#945;,16&amp;#946;, 26-hexol (1) and (25S)5&amp;#945;-cholestane-3&amp;#946;,6&amp;#945;,7&amp;#945;,8&amp;#946;,15&amp;#945;,16&amp;#946;,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ±0.00 and and 1.02 ± 0.01 &amp;#956;M). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. CONCLUSION: This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100012	
Editor: 	Sociedad de Biología de Chile	
Formato: 	text/html	
Fonte: 	Biological Research  v.48 2015