Data/hora: 09/06/2024 10:39:51

Provedor de dados: 	48	
País: 	Chile	
Título: 	In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA	
Autores: 	RIOS,MARIANA; VENEGAS,ALEJANDRO; CROXATTO,HORACIO B.	
Data: 	2002-01-01	
Ano: 	2002	
Palavras-chave: 	SS-galactosidase; Gene transfer; Oviduct.	
Resumo: 	Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (&lt;A HREF="#rios97"&gt;Ríos et al., 1997&lt;/A&gt;). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal plasmid increased ß-gal activity by 71% and 142%, respectively, over the control oviducts. These results indicate that naked DNA and mRNA coding for ß-gal can enter oviductal cells and be translated into an active enzyme. They are consistent with the interpretation that embryo transport acceleration caused by the injection of estradiol-induced RNA in the oviduct involves translation of the injected mRNA	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007	
Editor: 	Sociedad de Biología de Chile	
Formato: 	text/html	
Fonte: 	Biological Research  v.35 n.3-4 2002