Data/hora: 04/06/2024 06:45:05

Provedor de dados: | 48
País: | Chile
Título: | Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk
Autores: | SOUZA VIANA,ELISETH; MARTINO CAMPOS,MARIA EMILENE; REIS PONCE,ADRIANA; CUQUETTO MANTOVANI,HILÁRIO; DANTAS VANETTI,MARIA CRISTINA.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Acyl homoserines lactones; Biofilm; Furanones; Quorum sensing.
Resumo: | The objective of this study was to detect the presence of acyl homoserine lactones (AHLs), signal molecules of the quorum sensing system in biofilm formed by Hafnia alvei strains. It also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. AHLs were assayed using well diffusion techniques, thin layer chromatography (TLC) and detection directly in biofilm with biomonitors. The extracts obtained from planktonic and sessile cell of H. alvei induced at least two of three monitor strains evaluated. The presence of AHLs with up to six carbon atoms was confirmed by TLC. Biofilm formation by H. alvei was inhibited by furanone, as demonstrated by 96-well assay of crystal violet in microtitre plates and by scanning electron microscopy. The H. alvei 071 hall mutant was deficient in biofilm formation. All these results showed that the quorum sensing system is probably involved in the regulation of biofilm formation by H. alvei.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400004
Editor: | Sociedad de Biología de Chile
Formato: | text/html
Fonte: | Biological Research  v.42 n.4 2009