Data/hora: 29/05/2024 04:49:54

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	Molecular responses of Ostrea edulis haemocytes to an in vitro infection with Bonamia ostreae	
Autores: 	Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan.	
Data: 	2011-03	
Ano: 	2011	
Palavras-chave: 	Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation.	
Resumo: 	Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms.  Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in cytoskeleton, respiratory chain, detoxification membrane receptors, and immune system were identified. The open reading frames of two selected genes (galectin and IRF-like) were completely sequenced and characterized. Real time PCR assays were developed to study the relative expression of candidate ESTs during an in vitro infection of haemocytes by live and dead parasites.  Haemocyte infection with B. ostreae induced an increased expression of omega glutathione S-transferase (OGST), superoxide dismutase (SOD), tissue inhibitor of metalloproteinase (TIMP), galectin, interferon regulatory factor (IRF-like) and filamin genes.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00018/12879/9828.pdf

DOI:10.1016/j.dci.2010.10.005	
Editor: 	Elsevier Sci Ltd	
Relação: 	http://archimer.ifremer.fr/doc/00018/12879/	
Formato: 	application/pdf	
Fonte: 	Developmental And Comparative Immunology (0145-305X) (Elsevier Sci Ltd), 2011-03 , Vol. 35 , N. 3 , P. 323-333	
Fonte: 	2010 Elsevier Ltd All rights reserved