Data/hora: 08/06/2024 12:45:34

Provedor de dados: | 5
País: | France
Título: | A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen
Autores: | Mesiet Cladiere, L; Norais, Cédric; Kuhn, Joelle; Briffotaux, Julien; Sloostra, Jerry W.; Ferrari, Elena; Hubscher, Ulrich; Flament, Didier; Myllykallio, Hannu.
Data: | 2007-10
Ano: | 2007
Palavras-chave: | Ribonuclease HII; Combinatorial peptide synthesis; PCNA.
Resumo: | During DNA replication and repair, many proteins bind to and dissociate in a highly specific and ordered manner from proliferating cell nuclear antigen (PCNA). We describe a combined approach of in silico searches at the genome level and combinatorial peptide synthesis to investigate the binding properties of hundreds of short PCNA-interacting peptides (PIP-peptides) to archaeal and eukaryal PCNAs. Biological relevance of our combined approach was demonstrated by identification an inactive complex of Pyrococcus abyssi ribonuclease HII with PCNA. Furthermore we show that PIP-peptides interact with PCNA largely in a sequence independent manner. Our experimental approach also identified many so far unidentified PCNA interacting peptides in a number of human proteins.   (c) 2007 Elsevier Ltd. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2007/publication-3297.pdf

DOI:10.1016/j.jmb.2007.06.056

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/3297/
Formato: | application/pdf
Fonte: | Journal of Molecular Biology (0022-2836) (Elsevier), 2007-10 , Vol. 372 , N. 5 , P. 1137-1148
Fonte: | 2007 Elsevier Ltd All rights reserved