Data/hora: 08/06/2024 14:43:10

Provedor de dados: 	5	
País: 	France	
Título: 	Characterisation of the spoilage microbiota in raw salmon (Salmo salar) steaks stored under vacuum or modified atmosphere packaging combining conventional methods and PCR–TTGE	
Autores: 	Mace, Sabrina; Cornet, Josiane; Chevalier, Frederique; Cardinal, Mireille; Pilet, Marie-france; Dousset, Xavier; Joffraud, Jean-jacques.	
Data: 	2012-05	
Ano: 	2012	
Palavras-chave: 	Seafood; Culture-independent method (PCR-TTGE); Sensory analysis; Photobacterium phosphoreum; Lactococcus piscium.	
Resumo: 	In order to characterise the spoilage related to microbiota of raw salmon, a combination of culture-dependent and -independent methods, including PCR–TTGE, was used to analyse 3 raw salmon batches stored for 3 days at chilled temperature in modified atmosphere packaging (MAP) (50% CO2/50% N2) or under vacuum. Sensory evaluation, microbiological enumeration and chemical analysis were performed after 3, 7 and 10 days of storage. At the onset of spoilage, 65 bacterial isolates were picked from the plates. Thus, 13 different genera or species were identified by phenotypic and molecular tests: Serratia spp., Photobacterium phosphoreum, Yersinia intermedia, Hafnia alvei, Buttiauxella gaviniae, Pseudomonas sp., Carnobacterium maltaromaticum, Carnobacterium divergens, Lactococcus piscium, Lactobacillus fuchuensis, Vagococcus carniphilus, Leuconostoc gasicomitatum and Brochothrix thermosphacta. The PCR–TTGE profiles and band identification enabled a shift of the dominant populations during the storage to be visualised for all the batches, probably due to the temperature change and the packaging. At the beginning of storage, Pseudomonas sp. dominated the raw salmon microbiota while in the following days (7 and 10), P. phosphoreum and L. piscium were identified as the main bacterial groups. This study enhances the knowledge of MAP and vacuum-packed raw salmon spoilage microbiota.  Highlights  13 different bacterial taxa were identified by phenotypic and molecular tests. A shift of the dominant microflora during storage was brought to light. The dominant bacterial populations displayed by PCR–TTGE were identified.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00052/16277/13779.pdf

DOI:10.1016/j.fm.2011.10.013

https://archimer.ifremer.fr/doc/00052/16277/	
Editor: 	Academic Press Ltd- Elsevier Science Ltd	
Formato: 	application/pdf	
Fonte: 	Food Microbiology (0740-0020) (Academic Press Ltd- Elsevier Science Ltd), 2012-05 , Vol. 30 , N. 1 , P. 164-172	
Fonte: 	2011 Elsevier Ltd. All rights reserved.

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