Data/hora: 09/06/2024 05:45:24

Provedor de dados: 	5	
País: 	France	
Título: 	Archaeal communities associated with shallow to deep subseafloor sediments of the New Caledonia Basin	
Autores: 	Roussel, Erwan; Sauvadet, Anne-laure; Chaduteau, Carine; Fouquet, Yves; Charlou, Jean-luc; Prieur, Daniel; Cambon, Marie-anne.	
Data: 	2009-09	
Ano: 	2009	
Palavras-chave: 	Marine subsurface sediments; Sea floor biosphere; Gradient gel electrophoresis; Microbial communities; Molecular diversity; Anaerobic oxidation; Sequence alignment; Phylogenetic trees; Extracellular DNA; Methane hydrate.	
Resumo: 	P&gt;The distribution of the archaeal communities in deep subseafloor sediments [0-36 m below the seafloor (mbsf)] from the New Caledonia and Fairway Basins was investigated using DNA- and RNA-derived 16S rRNA clone libraries, functional genes and denaturing gradient gel electrophoresis (DGGE). A new method, Co-Migration DGGE (CM-DGGE), was developed to access selectively the active archaeal diversity. Prokaryotic cell abundances at the open-ocean sites were on average similar to 3.5 times lower than at a site under terrestrial influence. The sediment surface archaeal community (0-1.5 mbsf) was characterized by active Marine Group 1 (MG-1) Archaea that co-occurred with ammonia monooxygenase gene (amoA) sequences affiliated to a group of uncultured sedimentary Crenarchaeota. However, the anoxic subsurface methane-poor sediments (below 1.5 mbsf) were dominated by less active archaeal communities, such as the Thermoplasmatales, Marine Benthic Group D and other lineages probably involved in the methane cycle (Methanosarcinales, ANME-2 and DSAG/MBG-B). Moreover, the archaeal diversity of some sediment layers was restricted to only one lineage (Uncultured Euryarchaeota, DHVE6, MBG-B, MG-1 and SAGMEG). Sequences forming two clusters within the Thermococcales order were also present in these cold subseafloor sediments, suggesting that these uncultured putative thermophilic archaeal communities might have originated from a different environment. This study shows a transition between surface and subsurface sediment archaeal communities.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2009/publication-6801.pdf

DOI:10.1111/j.1462-2920.2009.01976.x	
Editor: 	Wiley / Blackwell	
Relação: 	http://archimer.ifremer.fr/doc/00000/6801/	
Formato: 	application/pdf	
Fonte: 	Environmental Microbiology (1462-2912) (Wiley / Blackwell), 2009-09 , Vol. 11 , N. 9 , P. 2446-2462	
Fonte: 	2009 Society for Applied Microbiology and Wiley - Blackwell Publishing Ltd.