Data/hora: 09/06/2024 13:46:38

Provedor de dados: | 5
País: | France
Título: | Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios
Autores: | Salin, Karine; Villasevil, Eugenia M.; Auer, Sonya K.; Anderson, Graeme J.; Selman, Colin; Metcalfe, Neil B.; Chinopoulos, Christos.
Data: | 2016-10
Ano: | 2016
Palavras-chave: | ATPase; Fluorescence; Magnesium green; Oxidative phosphorylation; Oxygraph.
Resumo: | The use of tissue homogenate has greatly aided the study of the functioning of mitochondria. However, the amount of ATP produced per oxygen molecule consumed, that is, the effective P/O ratio, has never been measured directly in tissue homogenate. Here we combine and refine existing methods previously used in permeabilized cells and isolated mitochondria to simultaneously measure mitochondrial ATP production (JATP) and oxygen consumption (JO(2)) in tissue homogenate. A major improvement over existing methods is in the control of ATPases that otherwise interfere with the ATP assay: our modified technique facilitates simultaneous measurement of the rates of uncorrected ATP synthesis and of ATP hydrolysis, thus minimizing the amount of tissue and time needed. Finally, we develop a novel method of calculating effective P/O ratios which corrects measurements of JATP and JO(2) for rates of nonmitochondrial ATP hydrolysis and respiration, respectively. Measurements of JATP and JO(2) in liver homogenates from brown trout (Salmo trutta) were highly reproducible, although activity declined once homogenates were 2h old. We compared mitochondrial properties from fed and food-deprived animals to demonstrate that the method can detect mitochondrial flexibility in P/O ratios in response to nutritional state. This method simplifies studies examining the mitochondrial bioenergetics of tissue homogenates, obviating the need for differential centrifugation or chemical permeabilization and avoiding the use of nonmitochondrial ATPase inhibitors. We conclude that our approach for characterizing effective P/O ratio opens up new possibilities in the study of mitochondrial function in very small samples, where the use of other methods is limited.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00414/52500/53300.pdf

DOI:10.14814/phy2.13007

https://archimer.ifremer.fr/doc/00414/52500/

Editor: | Wiley
Formato: | application/pdf
Fonte: | Physiological Reports (2051-817X) (Wiley), 2016-10 , Vol. 4 , N. 20 , P. e13007 (1-9)

Fonte:

2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

info:eu-repo/semantics/openAccess

restricted use