Data/hora: 14/06/2024 17:50:59

Provedor de dados: 	5	
País: 	France	
Título: 	Polymorphism at the ITS and NTS Loci of Perkinsus marinus isolated from cultivated oyster Crassostrea corteziensis in Nayarit, Mexico and Phylogentic relationship to P. marinus along the Atlantic Coast	
Autores: 	Escobedo-fregoso, C.; Arzul, Isabelle; Carrasco, Noelia; Gutierrez-rivera, J. N.; Llera-herrera, Raul; Vazquez-juarez, R.	
Data: 	2015-04	
Ano: 	2015	
Palavras-chave: 	Crassostrea corteziensis; Perkinsus marinus; Internal transcribed spacer; Non-internal transcribed spacer; Aquatic disease.	
Resumo: 	Prevalence of the protozoan Perkinsus spp. in the gills of the pleasure oyster Crassostrea corteziensis from two estuaries in Nayarit, Mexico, was measured. The protozoan was identified by PCR amplification of the internal transcribed spacer (ITS) region of the rDNA of Perkinsus spp. The pathogen was found in 92% of oysters from Boca de Camichín and 77% of oysters from Pozo Chino. ITS sequences characterized from C. corteziensis showed 96–100% similarity to Perkinsus marinus. The most frequent ITS sequence, (GenBank JQ266236) had 100% identity with the ITS locus of P. marinus from New Jersey, Maryland, South Carolina, and Texas, and the second most frequent observed sequence (GenBank JQ266240) was 100% identical to ITS sequences of P. marinus from New ersey, South Carolina, Louisiana, and Bahía Kino, Sonora, Mexico. The 14 sequences from the non-transcribed spacer (NTS) showed 98% similarity to P. marinus from Texas. The most frequent polymorphism identified was at nucleotide 446 of the ITS region, however, the NTS showed the highest nucleotide diversity, thereby suggesting that this region is suitable for genotype identification. Moreover, the most conserved ITS marker is better for species-specific diagnosis. Both the ITS and NTS sequences of P. marinus obtained from C. corteziensis were grouped in two clades, identifying two allelic variants of P. marinus	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00137/24811/22892.pdf

DOI:10.1111/tbed.12090

http://archimer.ifremer.fr/doc/00137/24811/	
Editor: 	Wiley-blackwell	
Formato: 	application/pdf	
Fonte: 	Transboundary And Emerging Diseases (1865-1674) (Wiley-blackwell), 2015-04 , Vol. 62 , N. 2 , P. 137-147	
Fonte: 	2013 Blackwell Verlag GmbH

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