Data/hora: 02/06/2024 01:01:07

Provedor de dados: 	5	
País: 	France	
Título: 	Exopolysaccharide biosynthesis and biodegradation by a marine hydrothermal Alteromonas sp. strain	
Autores: 	Lelchat, Florian; Cozien, Joelle; Le Costaouec, Tinaig; Brandily, Christophe; Schmitt, Sophie; Baudoux, Anne-claire; Colliec-jouault, Sylvia; Boisset, Claire.	
Data: 	2015-03	
Ano: 	2015	
Palavras-chave: 	Marine Alteromonas macleodii sp strain; Bacterial exopolysaccharides; Microbial extracellular enzymes; Endogenous enzymatic degradation.	
Resumo: 	Alteromonas macleodii subsp. fijiensis biovar deepsane is a deep-sea ecotype exopolysaccharide-producing bacteria isolated from the polychaete annelid Alvinella pompejana. The high molecular weight biopolymer HYD657 produced by this strain, is the first marine exopolysaccharide (EPS) to be commercialized for cosmetic use. Depolymerization methods are necessary to elucidate the complete structure of this EPS and to generate potentially bioactive oligosaccharides. Enzymatic methods are useful for elucidating polysaccharide structure because they specifically cleave glycosidic bonds and do not require harsh chemical conditions. The HYD657 EPS is structurally complex and no commercially available enzymes are able to effectively degrade it. Here, we present the first results on the endogenous enzymatic depolymerization of a marine EPS of biotechnological interest by the producing strain. Enzymatic activity was detected in the bacterial lysate and was able to decrease the apparent molecular size of the EPS, releasing mainly oligosaccharides. The reduced form of the native polysaccharide showed a slightly modified osidic composition, particularly in terms of molar ratio. Several exoglycosidase activities were measured in the bacterial lysate using paranitrophenyl-osides	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00240/35129/33893.pdf

DOI:10.1007/s00253-014-6075-y

https://archimer.ifremer.fr/doc/00240/35129/	
Editor: 	Springer	
Formato: 	application/pdf	
Fonte: 	Applied Microbiology And Biotechnology (0175-7598) (Springer), 2015-03 , Vol. 99 , N. 6 , P. 2637-2647	
Fonte: 	Springer-Verlag Berlin Heidelberg 2014

info:eu-repo/semantics/openAccess

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