Data/hora: 05/06/2024 10:43:11

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Lysozyme gene expression and hemocyte behaviour in the Mediterranean mussel, Mytilus galloprovincialis, after injection of various bacteria or temperature stresses
Autores: | Li, H; Parisi, M; Toubiana, Mylene; Cammarata, M; Roch, Philippe.
Data: | 2008-07
Ano: | 2008
Palavras-chave: | Molluscs; Mytilus; ISH; Q PCR; Gene expression; Lysozyme; Heat shock; Micrococcus lysodeikticus; Vibrio anguillarum; Vibrio splendidus.
Resumo: | The aim of the present study was to evaluate the expression of the Mytilus galloprovincialis lysozyme gene in different in vivo stress situations, including injection of bacteria Vibrio splendidus LGP32, Vibrio anguillarum or Micrococcus lysodeikticus, as well as heat shock at 30 C and cold stress at 5 C. Injection of V. splendidus LGP32 resulted in: (i) a general down-regulation of lysozyme gene expression, as quantified by Q-PCR; (ii) reduction in the number of circulating hemocytes; (iii) decrease in the percentage of circulating hemocytes expressing lysozyme mRNA which was now restricted to only small cells, as observed by ISH; and (iv) accumulation of hemocytes expressing lysozyme in the muscle sinus where injection took place. Injection of V. anguillarum or M. lysodeikticus induced significant up-regulation of lysozyme gene expression, but only 2-3 days post-injection, with no change in the total hemocyte counts but an increased percentage of hemocytes expressing lysozyme mRNA. Neither the control injection of PBS-NaCl nor temperature stress modified the lysozyme expression pattern. Consequently, the hemocyte population appears to be capable of discriminating between stress factors, and even between 2 Vibrio species. (C) 2008 Elsevier Ltd. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2008/publication-4479.pdf

DOI:10.1016/j.fsi.2008.04.001

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/4479/
Formato: | application/pdf
Fonte: | Fish &amp; Shellfish Immunology (1050-4648) (Elsevier), 2008-07 , Vol. 25 , N. 1-2 , P. 143-152
Fonte: | 2008 Elsevier Ltd All rights reserved.