Data/hora: 09/06/2024 04:16:38

Provedor de dados: 	5	
País: 	France	
Título: 	Transcriptomic features of Pecten maximus oocyte quality and maturation	
Autores: 	Pauletto, Marianna; Milan, Massimo; Huvet, Arnaud; Corporeau, Charlotte; Suquet, Marc; Planas, Josep V.; Moreira, Rebeca; Figueras, Antonio; Novoa, Beatriz; Patarnello, Tomaso; Bargelloni, Luca.	
Data: 	2017-03	
Ano: 	2017	
Resumo: 	The king scallop Pecten maximus is a high valuable species of great interest in Europe for both fishery and aquaculture. Notably, there has been an increased investment to produce seed for enhancement programmes of wild scallop populations. However, hatchery production is a relatively new industry and it is still underdeveloped. Major hurdles are spawning control and gamete quality. In the present study, a total of 14 scallops were sampled in the bay of Brest (Brittany, France) to compare transcriptomic profiles of mature oocytes collected by spawning induction or by stripping. To reach such a goal, a microarray analysis was performed by using a custom 8x60K oligonucleotide microarray representing 45,488 unique scallop contigs. First we identified genes that were differentially expressed depending on oocyte quality, estimated as the potential to produce D-larvae. Secondly, we investigated the transcriptional features of both stripped and spawned oocytes. Genes coding for proteins involved in cytoskeletal dynamics, serine/threonine kinases signalling pathway, mRNA processing, response to DNA damage, apoptosis and cell-cycle appeared to be of crucial importance for both oocyte maturation and developmental competence. This study allowed us to dramatically increase the knowledge about transcriptional features of oocyte quality and maturation, as well as to propose for the first time putative molecular markers to solve a major bottleneck in scallop aquaculture.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00374/48484/48751.pdf

https://archimer.ifremer.fr/doc/00374/48484/48752.pdf

https://archimer.ifremer.fr/doc/00374/48484/48754.fasta

https://archimer.ifremer.fr/doc/00374/48484/48755.csv

https://archimer.ifremer.fr/doc/00374/48484/48756.csv

https://archimer.ifremer.fr/doc/00374/48484/48757.csv

https://archimer.ifremer.fr/doc/00374/48484/48758.csv

https://archimer.ifremer.fr/doc/00374/48484/48759.csv

DOI:10.1371/journal.pone.0172805

https://archimer.ifremer.fr/doc/00374/48484/	
Editor: 	Public Library Science	
Relação: 	info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED	
Formato: 	application/pdf	
Fonte: 	Plos One (1932-6203) (Public Library Science), 2017-03 , Vol. 12 , N. 3 , P. e0172805 (1-22)	
Fonte: 	2017 Pauletto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

info:eu-repo/semantics/openAccess

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