Data/hora: 11/06/2024 07:51:39

Provedor de dados: | 5
País: | France
Título: | A simple ATAC-seq protocol for population epigenetics
Autores: | De Carvalho Augusto, Ronaldo; Rey, Oliver; Cosseau, Céline; Chaparro, Cristian; Vidal-dupiol, Jeremie; Allienne, Jean-françois; Duval, David; Pinaud, Silvain; Tönges, Sina; Andriantsoa, Ranja; Luquet, Emilien; Aubret, Fabien; Dia Sow, Mamadou; David, Patrice; Thomson, Vicki; Joly, Dominique; Gomes Lima, Mariana; Federico, Déborah; Danchin, Etienne; Minoda, Aki; Grunau, Christoph.
Data: | 2020
Ano: | 2020
Palavras-chave: | Epigenetics; Epigenomics; ATAC-seq; Daphnia pulex; Schistosoma mansoni.
Resumo: | We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies, and the analysis of alignment results. We show that the protocol can be used to monitor chromatin structure changes in populations when exposed to environmental cues. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size (~10,000 cells), and it requires little molecular biology handling expertise.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00681/79353/81859.pdf

DOI:10.12688/wellcomeopenres.15552.2

https://archimer.ifremer.fr/doc/00681/79353/

Editor: | F1000 Research Ltd
Formato: | application/pdf
Fonte: | Wellcome Open Research (2398-502X) (F1000 Research Ltd), 2020 , Vol. 5 , P. 121 (24p.)

Fonte:

info:eu-repo/semantics/openAccess

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