Data/hora: 11/06/2024 04:30:24

Provedor de dados: | 5
País: | France
Título: | Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range
Autores: | Massa, Sonia I.; Pearson, Gareth A.; Aires, Tania; Kube, Michael; Olsen, Jeanine L.; Reinhardt, Richard; Serrao, Ester A.; Arnaud Haond, Sophie.
Data: | 2011-09
Ano: | 2011
Palavras-chave: | EST library; High temperature stress; Abiotic stress response; Climate change.
Resumo: | Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera midi during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30 min, 2 h, 4 h and 24 h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as 'molecular chaperones' and were over-expressed following heat-shock, while 12 (23.5%) were 'photosynthesis TUGs' generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30 min AHS library, followed by a slow decrease during further recovery. In contrast, 'photosynthesis TUGs' were under-expressed 30 min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24 h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified. (C) 2011 Elsevier B.V. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00044/15559/13326.pdf

DOI:10.1016/j.margen.2011.04.003

Editor: | Elsevier Science Bv
Relação: | http://archimer.ifremer.fr/doc/00044/15559/
Formato: | application/pdf
Fonte: | Marine Genomics (1874-7787) (Elsevier Science Bv), 2011-09 , Vol. 4 , N. 3 , P. 181-188
Fonte: | 2011 Elsevier B.V. All rights reserved.