Data/hora: 02/06/2024 06:45:09

Provedor de dados: | 5
País: | France
Título: | Gene-centromere mapping in meiotic gynogenetic European seabass
Autores: | Oral, Munevver; Colleter, Julie; Bekaert, Michhttps://w3.ifremer.fr/archimer-admin/author.jsp#ael; Taggart, John B.; Palaiokostas, Christos; Mcandrew, Brendan J.; Vandeputte, Marc; Chatain, Beatrice; Kuhl, Heiner; Reinhardt, Richard; Peruzzi, Stefano; Penman, David J.
Data: | 2017-06
Ano: | 2017
Palavras-chave: | Dicentrarchus labrax; Meiotic gynogenesis; Isogenic lines; DdRAD seq; Genetic map; Gene-Centromere map; Aquaculture.
Resumo: | Background Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families. Results From a single meiotic gynogenetic family consisting of 79 progeny, 42 million sequencing reads (Illumina, trimmed to 148 bases) resolved 6866 unique RAD-tags. The 340 male-informative SNP markers that were identified confirmed the lack of paternal contribution. A gene-centromere map was constructed based on 804 female-informative SNPs in 24 linkage groups (2n = 48) with a total length of 1251.02 cM (initial LG assignment was based on the seabass genome assembly, dicLab v1). Chromosome arm structure could be clearly discerned from the pattern of heterozygosity in each linkage group in 18 out of 24 LGs: the other six showed anomalies that appeared to be related to issues in the genome assembly. Conclusion Genome-wide screening enabled substantive verification of the production of the gynogenetic family used in this study. The large number of telomeric and subtelomeric markers with high heterozygosity values in the meiotic gynogenetic family indicate that such markers could be used to clearly distinguish between meiotic and mitotic gynogenetics.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00388/49901/50462.pdf

https://archimer.ifremer.fr/doc/00388/49901/50463.csv

https://archimer.ifremer.fr/doc/00388/49901/50464.csv

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https://archimer.ifremer.fr/doc/00388/49901/50468.fasta

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https://archimer.ifremer.fr/doc/00388/49901/50470.csv

https://archimer.ifremer.fr/doc/00388/49901/50471.csv

DOI:10.1186/s12864-017-3826-z

https://archimer.ifremer.fr/doc/00388/49901/

Editor: | Biomed Central Ltd
Relação: | info:eu-repo/grantAgreement/EC/FP7/262336/EU//AQUA EXCEL
Formato: | application/pdf
Fonte: | Bmc Genomics (1471-2164) (Biomed Central Ltd), 2017-06 , Vol. 18 , N. 1 , P. 449 (1-12)

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