Data/hora: 17/06/2024 15:02:08

Provedor de dados: 	5	
País: 	France	
Título: 	Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities	
Autores: 	Balti, Rafik; Le Balc'H, Romain; Brodu, Nicolas; Gilbert, Marthe; Le Gouic, Benjamin; Le Gall, Sophie; Sinquin, Corinne; Masse, Anthony.	
Data: 	2018-11	
Ano: 	2018	
Palavras-chave: 	Membrane filtration; Exopolysaccharides; Microalga; Concentration; Diafiltration.	
Resumo: 	Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 µm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L.h-1. m-2 were obtained during the concentration at 4 bar for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m.s-1; 49.7 L.h-1. m-2 for the diafiltration at 3.3 m.s-1. Permeate fluxes were correctly predicted from polysaccharide concentrations (10 % deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or time of filtration could be modulated following the cross-flow velocity. Polysaccharides were concentrated 6.3 to 10.4 times in such a way that the final sugars concentration reached 1.74 to 2.26 g.L-1. Rheological behavior of filtered solutions changed following the concentration progress. More than 80 % (w/w) of polysaccharides were recovered while 49 % and 99 % of proteins and salts were removed respectively. The filtrations allowed reaching a final monosaccharide content of dry matter equal to 48.9 % against 0.6 % (w/w) initially.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00445/55694/57360.pdf

DOI:10.1016/j.procbio.2018.06.021

https://archimer.ifremer.fr/doc/00445/55694/	
Editor: 	Elsevier Sci Ltd	
Formato: 	application/pdf	
Fonte: 	Process Biochemistry (1359-5113) (Elsevier Sci Ltd), 2018-11 , Vol. 74 , P. 175-184	
Fonte: 	2018 Elsevier Ltd. All rights reserved.

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