Data/hora: 02/06/2024 15:54:18

Provedor de dados: 	5	
País: 	France	
Título: 	Identification of Polyunsaturated Fatty Acids Synthesis Pathways in the Toxic Dinophyte Alexandrium minutum Using 13C-Labelling	
Autores: 	Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Lambert, Christophe; Bideau, Antoine; Bidault, Adeline; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe.	
Data: 	2020-10	
Ano: 	2020	
Palavras-chave: 	PUFA synthesis; 22:6n-3; DHA; PKS pathway; Dinophyte; Alexandrium minutum; Encystment.	
Resumo: 	The synthetic pathways responsible for the production of the polyunsaturated fatty acids 22:6n-3 and 20:5n-3 were studied in the Dinophyte Alexandrium minutum. The purpose of this work was to follow the progressive incorporation of an isotopic label (13CO2) into 11 fatty acids to better understand the fatty acid synthesis pathways in A. minutum. The Dinophyte growth was monitored for 54 h using high-frequency sampling. A. minutum presented a growth in two phases. A lag phase was observed during the first 30 h of development and had been associated with the probable temporary encystment of Dinophyte cells. An exponential growth phase was then observed after t30. A. minutum rapidly incorporated 13C into 22:6n-3, which ended up being the most 13C-enriched polyunsaturated fatty acid (PUFA) in this experiment, with a higher 13C atomic enrichment than 18:4n-3, 18:5n-3, 20:5n-3, and 22:5n-3. Overall, the 13C atomic enrichment (AE) was inversely proportional to number of carbons in n-3 PUFA. C18 PUFAs, 18:4n-3, and 18:5n-3, were indeed among the least 13C-enriched FAs during this experiment. They were assumed to be produced by the n-3 PUFA pathway. However, they could not be further elongated or desaturated to produce n-3 C20-C22 PUFA, because the AEs of the n-3 C18 PUFAs were lower than those of the n-3 C20-C22 PUFAs. Thus, the especially high atomic enrichment of 22:6n-3 (55.8% and 54.9% in neutral lipids (NLs) and polar lipids (PLs), respectively) led us to hypothesize that this major PUFA was synthesized by an O2-independent Polyketide Synthase (PKS) pathway. Another parallel PKS, independent of the one leading to 22:6n-3, was also supposed to produce 20:5n-3. The inverse order of the 13C atomic enrichment for n-3 PUFAs was also suspected to be related to the possible β-oxidation of long-chain n-3 PUFAs occurring during A. minutum encystment	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00654/76562/77688.pdf

https://archimer.ifremer.fr/doc/00654/76562/77689.zip

DOI:10.3390/biom10101428

https://archimer.ifremer.fr/doc/00654/76562/	
Editor: 	MDPI AG	
Formato: 	application/pdf	
Fonte: 	Biomolecules (2218-273X) (MDPI AG), 2020-10 , Vol. 10 , N. 10 , P. 1428 (21p.)	
Fonte: 	info:eu-repo/semantics/openAccess

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