Data/hora: 11/06/2024 01:26:38

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas
Autores: | Corporeau, Charlotte; Groisillier, Agnes; Jeudy, Alexandra; Barbeyron, Tristan; Fleury, Elodie; Fabioux, Caroline; Czjzek, Mirjam; Huvet, Arnaud.
Data: | 2011-10
Ano: | 2011
Palavras-chave: | Transforming growth factor-beta; Bacterial expression; In vivo antibody inhibition; Reproduction; Crassostrea gigas; Marine bivalve.
Resumo: | The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a rapid bacterial production system to obtain recombinant og TGF-beta protein, and we demonstrate that og TGF-beta is processed by furin to a mature form of the protein. This mature form can be detected in vivo in the gonad. Functional inhibition of mature og TGF-beta in the gonad was conducted by inactivation of the protein using injection of antibodies. We show that inhibition of og TGF-beta function tends to reduce gonadic area. We conclude that mature og TGF-beta probably functions as an activator of germ cells development in oyster.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00042/15371/13438.pdf

DOI:10.1007/s10126-010-9361-4

Editor: | Springer
Relação: | http://archimer.ifremer.fr/doc/00042/15371/
Formato: | application/pdf
Fonte: | Marine Biotechnology (1436-2228) (Springer), 2011-10 , Vol. 13 , N. 5 , P. 971-980
Fonte: | Springer Science+Business Media, LLC 2011