Data/hora: 10/06/2024 13:41:05

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant?
Autores: | Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R.
Data: | 2012-08
Ano: | 2012
Palavras-chave: | Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance.
Resumo: | The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and increases with DNA target length. It was established that k(2) (s(-1)), relevant to irreversible formation of a stable duplex, varies in an opposite way to K-1 with DNA target length. (C) 2012 Published by Elsevier B.V.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00101/21195/19415.pdf

DOI:10.1016/j.snb.2012.05.023

Editor: | Elsevier Science Sa
Relação: | http://archimer.ifremer.fr/doc/00101/21195/
Formato: | application/pdf
Fonte: | Sensors And Actuators B-chemical (0925-4005) (Elsevier Science Sa), 2012-08 , Vol. 171 , P. 522-527
Fonte: | 2012 Published by Elsevier B.V.