Data/hora: 09/06/2024 14:42:23

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia : Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCO genes	
Autores: 	Duperron, Sebastien; Fiala Medioni, Aline; Caprais, Jean-claude; Olu, Karine; Sibuet, Myriam.	
Data: 	2007	
Ano: 	2007	
Palavras-chave: 	Eastern Mediterranean; Cold seeps; Lucinoma; Lucinidae; Sulphide oxidizing bacteria; Symbiosis.	
Resumo: 	Symbioses between lucinid clams (Bivalvia: Lucinidae) and autotrophic sulphide-oxidizing bacteria have mainly been studied in shallow coastal species, and information regarding deep-sea species is scarce. Here we study the symbiosis of a clam, resembling Lucinoma kazani, which was recently collected in sediment cores from new cold-seep sites in the vicinity of the Nile deep-sea fan, eastern Mediterranean, at depths ranging from 507 to 1691 m. A dominant bacterial phylotype, related to the sulphide-oxidizing symbiont of Lucinoma aequizonata, was identified in gill tissue by comparative 16S rRNA gene sequence analysis. A second phylotype, related to spirochete sequences, was identified twice in a library of 94 clones. Comparative analyses of gene sequences encoding the APS reductase alpha subunit and ribulose-1,5-bisphosphate carboxylase oxygenase support the hypothesis that the dominant symbiont can perform sulphide oxidation and autotrophy. Transmission electron micrographs of gills confirmed the dominance of sulphide-oxidizing bacteria, which display typical vacuoles, and delta C-13 values measured in gill and foot tissue further support the hypothesis for a chemoautotrophic-sourced host carbon nutrition.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2007/publication-2176.pdf

DOI:10.1111/j.1574-6941.2006.00194.x	
Editor: 	Blackwell science	
Relação: 	http://archimer.ifremer.fr/doc/00000/2176/	
Formato: 	application/pdf	
Fonte: 	FEMS Microbiology Ecology (0168-6496) (Blackwell science), 2007 , Vol. 59 , N. 1 , P. 64-70	
Fonte: 	2007 Blackwell Publishing, Inc.