Data/hora: 04/06/2024 01:40:12

Provedor de dados: | 5
País: | France
Título: | The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA
Autores: | Chen, Meilin; Kuo, S. T.; Renault, Tristan; Chang, P. H.
Data: | 2014-02
Ano: | 2014
Palavras-chave: | Herpesvirus; Abalone; Haliotis diversicolor supertexta; Polymerase chain reaction; Loop-mediated isothermal amplification; SYBR green PCR.
Resumo: | A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63 °C and 60 min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10 folds less sensitive than a SYBR Green PCR. These results indicate that the developed LAMP assay is a simple, rapid, sensitive, specific and reliable technique for the detection of abalone herpesvirus.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00166/27690/25882.pdf

DOI:10.1016/j.jviromet.2013.11.011

https://archimer.ifremer.fr/doc/00166/27690/

Editor: | Elsevier Science Bv
Formato: | application/pdf
Fonte: | Journal Of Virological Methods (0166-0934) (Elsevier Science Bv), 2014-02 , Vol. 197 , P. 199-203

Fonte:

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