Data/hora: 08/06/2024 02:07:41

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Bacterial environment of gametes and turbot (Scophthalmus maximus) eggs.
Autores: | Omnes, Marie-helene; Temey, Yann; Daniel, Jean-yves; Nicolas, Jean-louis.
Data: | 1993-05
Ano: | 1993
Resumo: | Bacterial infections have been suspected to be one cause of variable success in the incubation of turbot (Scophthalmus maximus) eggs. The assessment of bacterial flora (bacterial environment) associated with ova, sperm and developing eggs was conducted just at stripping gametes and during incubation. Gametes were collected in sterile conditions before fertilization. Eggs were held in an open-circulation 401 incubator, at 13 degree C during 6 days until hatching. Samples of those were collected every day. They were rinsed several times with sterile seawater, counted and grown. Viable counts of bacteria were determined on two media, Zobell and TCBS agar. Epibiotic bacteria on the surface of the eggs and impact on the chorion were observed at scanning electron microscope. High variability in counts of bacteria was observed between samples. Ova and sperm were both, intra-ovularly and intra-spermiductly infected (from &lt;10 to 10 super(3) bact./ova; from &lt;10 to 10 super(6) bact./ml sperm). Over the developmental stages, bacteria flora was associated with eggs (from 10 to 10 super(4) bact./egg), and was present in the incubator medium (10 super(3)-10 super(4) bact./ml seawater). Phenotypic identification of bacteria revealed a diversified bacterial flora associated with incubated eggs. The hatching rates achieved variable levels (from 15% to 46% viable larvae), but no correlation was found between number of bacteria and losses of eggs.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00199/31015/29425.pdf

http://archimer.ifremer.fr/doc/00199/31015/

Editor: | World Aquaculture '93 International Conférence, 26-28 May 1993, Torremolinos (Spain)
Formato: | application/pdf

Fonte:

info:eu-repo/semantics/openAccess

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