Data/hora: 12/06/2024 12:39:40

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Evaluation by molecular biology of enterovirus and hepatitis A virus contamination of shellfish in the Loire River estuary region
Autores: | Leguyader, F; Apaire, M; Besse, B; Billaudel, S.
Data: | 1992-04
Ano: | 1992
Palavras-chave: | France; Loire R. ANE; France; Pays de Loire; Loire Estuary Hepatitis A virus Enterovirus Bivalvia Hybridization analysis Seafood Estuaries Rivers Sensors RNA Disease detection Viruses Shellfish Biology.
Resumo: | Riboprobes developed in our laboratory were used for detection of enteroviruses and hepatitis A virus (HAV) in shellfish (cockles and mussels). After concentration elution, virus nucleic acids were extracted and purified. Hybridization was performed on a solid support according to the dot-blot technique, with (32P) UTP labelling of probes during transcription. Results show high shellfish contamination in samples: 63% enteroviruses and 67% HAV. Cockles were particularly contaminated: 89% enteroviruses and 84% HAV.
Tipo: | Text
Idioma: | Inglês
Identificador: | http://archimer.ifremer.fr/doc/1992/acte-1616.pdf
Editor: | Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1995
Relação: | http://archimer.ifremer.fr/doc/00000/1616/
Formato: | application/pdf