Data/hora: 02/06/2024 06:25:09

Provedor de dados: | 5
País: | France
Título: | Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas
Autores: | Bouilly, Karine; Leitao, Alexandra; Chaves, Raquel; Guedes Pinto, Henrique; Boudry, Pierre; Lapegue, Sylvie.
Data: | 2005-02
Ano: | 2005
Palavras-chave: | Crassostrea gigas; Pacific oyster; Restriction enzyme digestion chromosome banding; Atrazine; Aneuploidy; Crassostrea gigas; Huître creuse; Marquage chromosomique par digestion d'enzymes de restriction; Atrazine; Aneuploïdie.
Resumo: | Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were analysed using the restriction enzyme HaeIII. The study of 26 RE-banded aneuploid karyotypes showed that the same chromosome pairs (1, 5, 9, and 10) were affected by the loss of 1 chromosome (61%, 15%, 42%, and 42%, respectively). Further investigation is required to enable a better understanding of aneuploidy in oysters, especially with respect to why some chromosomes are more easily lost than others, and why cells tolerate the loss of these chromosomes.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/2005/publication-654.pdf

DOI:10.1139/G04-087

https://archimer.ifremer.fr/doc/00000/654/

Editor: | NRC
Formato: | application/pdf
Fonte: | Genome (0831-2796) (NRC), 2005-02 , Vol. 48 , N. 1 , P. 177-180

Fonte:

2005 National Research Council of Canada

info:eu-repo/semantics/openAccess

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