Data/hora: 04/06/2024 15:31:45

Provedor de dados: 	5	
País: 	France	
Título: 	Detection of cytotoxin-hemolysin mRNA in nonculturable populations of environmental and clinical Vibrio vulnificus strains in artificial seawater	
Autores: 	Fischer Le Saux, Marion; Hervio Heath, Dominique; Loaec, Solen; Colwell, Rita; Pommepuy, Monique.	
Data: 	2002-11	
Ano: 	2002	
Palavras-chave: 	Artificial seawater; Reverse transcription PCR; Cytotoxin Hemolysin; Molecular detection method; Vibrio vulnificus.	
Resumo: 	The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvhA. Three strains, two environmental, IF Vv10 and IF Vv18, and one clinical, C7184, were used in this study. Artificial seawater, inoculated with mid-log-phase cells, was maintained at 4degreesC. VBNC cells resulted after 3, 6, and 14 days for C7184, IF Vv18, and IF Vv10, respectively. Our data indicate that seminested RT-PCR is sensitive for the detection of vvhA mRNA in artificial seawater when exclusively nonculturable bacteria are present. This is the first report of the expression of a toxin gene in VBNC V. vulnificus. Moreover, vvhA transcripts were shown to persist in nonculturable populations over a 4.5-month period, with a progressive decline of the signal over time. This result indicates that special attention should be given to the presence of potentially pathogenic VBNC cells in environmental samples when assessing public health risk.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/2002/publication-1269.pdf

DOI:10.1128/AEM.68.11.5641-5646.2002

https://archimer.ifremer.fr/doc/00000/1269/	
Editor: 	American society for microbiology	
Formato: 	application/pdf	
Fonte: 	Applied and environmental microbiology (0099-2240) (American society for microbiology), 2002-11 , Vol. 68 , N. 11 , P. 5641-5646	
Fonte: 	2002, American Society for Microbiology. All Rights Reserved

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