Data/hora: 13/06/2024 02:32:58

Provedor de dados: | 5
País: | France
Título: | Exoproteolytic activity determined by flow injection analysis: its potential importance for bacterial growth in coastal marine ponds
Autores: | Delmas, Daniel; Legrand, Catherine; Bechemin, Christian; Collinot, Cecile.
Data: | 1994
Ano: | 1994
Palavras-chave: | Activité exoprotéolytique; Protéine dissoute; Analyse en flux continu; Bactérioplancton; Marais maritime; Flow injection analysis; Exoproteolytic activity; Dissolved protein; Bacterioplankton; Coastal marine pond.
Resumo: | The measurement of the fluorescent 4-methyl-7-coumarinylamine released from the hydrolysis of a non-fluorescent peptide model substrate by exoproteolytic enzymes has been adapted to flow injection analysis (FIA). FIA allows samples to be processed very quickly (less than 2 min. for triplicate determinations) with good sensitivity (&lt; 0.1 muM) and reproducibility (relative standard deviation &lt; 3 % at the 0.2 muM level). In a coastal marine pond, exoproteolytic activity was closely related to bacterioplankton biomass. The high activity measured in pond water (maximum velocity: V(M) almost-equal-to 1.46 to 2.54 muM.h-1) emphasizes the importance of dissolved protein hydrolysis for bacterial growth and for dissolved organic nitrogen cycling. The mean turnover time of dissolved peptides was 7.6 days, and amino acids liberated by exoproteolytic activity could potentially support, on average, 40 % of the bacterial nitrogen demand.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00196/30756/29163.pdf

DOI:10.1051/alr:1994003

Editor: | Gauthier-villars
Relação: | http://archimer.ifremer.fr/doc/00196/30756/
Formato: | application/pdf
Fonte: | Aquatic Living Resources (0990-7440) (Gauthier-villars), 1994 , Vol. 7 , N. 1 , P. 17-24
Fonte: | IFREMER-Gauthier-Villars