Data/hora: 10/06/2024 08:18:23

Provedor de dados: | 5
País: | France
Título: | Effect of temperature, volume of ova batches, and addition of a diluent, an antibiotic, oxygen and a protein inhibitor on short-term storage capacities of turbot, Psetta maxima, ova
Autores: | Suquet, Marc; Chereguini, Olvido; Omnes, Marie-helene; Rasines, Inmaculada; Normant, Yvon; Souto, Isabel; Quemener, Loic.
Data: | 1999-07
Ano: | 1999
Palavras-chave: | Psetta maxima; Turbot; Calcium; Ovarian fluid; Short term storage; Ova; Fish; Psetta maxima; Turbot; Calcium; Liquide ovarien; Conservation à court terme; Ovules; Poisson.
Resumo: | The effect of different parameters on shea-term storage capacity of turbot ova was assessed over a 45-h period after ova collection for fertilization rates and over a 9-h period after ova collection for hatching rates. Increasing the volume of ova sampling from 0.5 to 2.5 mt, as well as adding an antibiotic-antimicotic solution or oxygen did not significantly change the storage capacity of ova. Regarding the hatching rates, a higher storage ability was recorded at 8 and 13 degrees C, compared to 3 degrees C. The mean composition of the ovarian fluid was determined (n = 57 spawns). Use of a diluent mimicking the ovarian fluid significantly decreased the storage ability as assessed by the fertilization rates but did not modify the hatching rates. Diluting ova in an artificial ovarian fluid deprived of calcium significantly decreased the fertilization and hatching rates during the storage period. Furthermore, addition or not of soybean trypsin inhibitor (Sigma T 9003) to the artificial ovarian fluid deprived of calcium did not significantly change the results. Storage capacity of control batches of ova was low: at 13 degrees C, without any diluent and when ova were fertilized 3 h after stripping, the hatching rate was lowered to 62.4 +/- 29.4 % (mean +/- SD) of the initial value.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/1999/publication-857.pdf

DOI:10.1016/S0990-7440(00)86634-6

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/857/
Formato: | application/pdf
Fonte: | Aquatic Living Resources (0990-7440) (Elsevier), 1999-07 , Vol. 12 , N. 4 , P. 239-246
Fonte: | 1999 Ifremer/Cnrs/Inra/Ird/Cemagref/Éditions scientifiques et médicales Elsevier SAS