Data/hora: 05/06/2024 00:36:05

Provedor de dados: 	Institutional Archive of Ifreme (ArchiMer)	
País: 	France	
Título: 	Two Vibrio splendidus related strains collaborate to kill Crassostrea gigas: taxonomy and host alterations	
Autores: 	Gay, Melanie; Renault, Tristan; Pons, Anne-marie; Le Roux, Frédérique.	
Data: 	2004-11	
Ano: 	2004	
Palavras-chave: 	Histopathology; Molecular taxonomy; Agonism; Vibriosis.	
Resumo: 	For several years, strains phenotypically related to Vibrio splendidus have been associated with mortality outbreaks of molluscs. A former study on Crassostrea gigas demonstrated the genetic diversity of V splendidus strains associated with diseased animals. Another study suggested that different strains may act in an additive/synergistic way leading to higher C. gigas mortality rates. Here, a strain pair (31+32) was characterised at taxonomic and virulence levels. Using a polyphasic approach, these strains were confirmed to be V splendidus-related, without a clear discrimination between V. kanaloae and V. pomeroyi since hybridisation rates with both these strains were above 70%. Following experimental infection of C. gigas by injection in the adductor muscle or in the pallial cavity, the host alterations induced were described. After injection of strains 31 and/or 32, bacteria were localised at the periphery of the muscle and induced extensive lesions of the translucent part of the adductor muscle. Muscle alterations were of 3 kinds: (1) presence of isolated rounded muscular fibres containing non-homogenous granular material and surrounded by a translucent halo; (2) presence of non-homogenous granular material in the cytoplasm of entire muscle bands; (3) affection of wide muscle areas with extremely condensed muscle fibres. Infiltration associated with these lesions was notably absent in the vast majority of the individuals.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2004/publication-2912.pdf	
Editor: 	Inter-Research	
Relação: 	http://archimer.ifremer.fr/doc/00000/2912/	
Formato: 	application/pdf	
Fonte: 	Diseases of aquatic organisms (0177-5103) (Inter-Research), 2004-11 , Vol. 62 , N. 1-2 , P. 65-74	
Fonte: 	Inter-Research