Data/hora: 01/06/2024 15:20:59

Provedor de dados: | Institutional Archive of Ifreme (ArchiMer)
País: | France
Título: | Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas
Autores: | Da Costa Gonzalez, Fiz; Robert, Rene; Quere, Claudie; Wikfors, Gary H.; Soudant, Philippe.
Data: | 2015-05
Ano: | 2015
Palavras-chave: | Fatty acid; Larvae; Lipids; Metabolism; Oyster; Synthesis.
Resumo: | Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5 desaturase. Moreover, this Delta 5 desaturase presumably allowed larvae to convert 20:3n-6 and 20:4n-3 to 20:4n-6 and 20:5n-3, respectively, when the product EFA were poorly or not supplied in the diet, as when larvae were fed T exclusively. Under our experimental conditions, none of the diets induced 22:6n-3 synthesis; however, 22:6n-3 incorporation into larval tissues occurred selectively under non-limiting dietary supply to maintain optimal levels in the larvae. This combination of flow-through larval rearing and biochemical analysis of FA levels could be applied to additional dietary experiments to precisely define optimal levels of EFA supply.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00268/37962/36086.pdf

DOI:10.1007/s11745-015-4006-z

https://archimer.ifremer.fr/doc/00268/37962/

Editor: | Springer Heidelberg
Formato: | application/pdf
Fonte: | Lipids (0024-4201) (Springer Heidelberg), 2015-05 , Vol. 50 , N. 5 , P. 503-511

Fonte:

AOCS 2015

info:eu-repo/semantics/openAccess

restricted use